Ultraviolet visible spectroscopy (UV-VIS)

Ultraviolet visible spectroscopy (UV-Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum, molecules undergo electronic transitions.


UV/VIS spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macro molecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.