Dr. Rosy Mondal
Head and neck cancer is the most commonly diagnosed cancer worldwide, with 90%–95% of the cases reported to be squamous cell carcinoma. The disease is one of the most mystifying, commonly misdiagnosed and poorly understood entities because the lesion is often situated in a relatively inert space where only air and mucus are in transit. Head and neck squamous cell carcinoma (HNSCC) can be silent for a long time without any primary symptoms. In Northeast India, HNSCC is most familiar in the states of Assam, Manipur, Mizoram, Tripura, and Nagaland with an incidence of 54.48%, which acquires 30%–40% cancers at all sites and is the sixth most common cause of death in males and seventh in females.
Our current research is focused on circulating cell-free nucleic acid.The clinical viewpoint of cell-free DNA (cfDNA) has sparked the interest of scientists as it opens up a new possibility for non-invasive analysis of tumor-derived genetic materials such as circulating tumor cells (CTCs), cfDNA, cell-free mitochondrial DNA (cfmtDNA), circulating RNA, or microRNAs. The idea of a “liquid biopsy” analysis may provide a means for non-invasive, real-time monitoring, with potential applications of prognosis, response to therapy, and emergence of treatment resistance.
The overall goal of this proposal is to detect the presence and fractions of circulating cell-free tumour DNA (ccftDNA) in plasma of cancer patients and determine the feasibility of a deep sequencing-based [exome] approach to cancer detection.
Post-Doctoral Research Associate, Boston University School of Medicine, 2013 – 2014
Ph.D. (Biotechnology), Assam University (A Central University), Silchar, Assam. 2009 – 2013
M.Sc. Biotechnology, Periyar University, Tamil Nadu. 2005 – 2007
B.Sc. (Hons) Biotechnology, Burdwan University, West Bengal. 2002-2005
|Institute of Advanced Study in Science & Technology, Guwahati||DST INSPIRE Faculty||-2015||Till date|
College and Hospital, Silchar, Assam
|Research Scientist II||2014||2015|
|Boston University ,School of Medicine, Boston, MA, USA||Postdoctoral Research Associate||07.02.2014||31.07.2014|
|Assam University, Silchar||Research Assistant||2013||2014|
|Cyber Research and Training Institute, affiliated to Burdwan University, West Bengal||Senior Faculty||2007||2008|
- Kumar, M., Sarma, M.K., Choudhury, Y., Ghosh, S.K., Mondal, R*.(2019) Somatic Mutations in Circulating Cell-Free DLoop Region of Mitochondrial DNA: A Study from NorthEast India. Journal of Biological Engineering Research and Review , 6(1)(*corresponding author)
- Kumar, M., Choudhury, Y., Ghosh, S.K., Mondal, R.*(2018). Application and optimization of minimally invasive cell-free DNA techniques in oncogenomics. Tumor Biology, 40(2) (*corresponding author)
- Choudhury, J.H., Das, R., Laskar, S., Kundu, S., Kumar, M., Das, P.P., Choudhury, Y., Mondal, R., Ghosh, S.K. (2018) Detection of p16 promoter hypermethylation by methylation-specific PCR. Methods in Molecular Biology, 1726, pp. 111-122. DOI: 10.1007/978-1-4939-7565-5_11
- Kumar M, Srivastava S, Singh A, Das AK, Das GC., Dhar B, Ghosh SK, Mondal R. (2017). Cell-free mitochondrial DNA copy number variation in head and neck squamous cell carcinoma: A study of non-invasive biomarker from Northeast India. Tumor Biology, 39 (10), pp. 1-9. DOI: 10.1177/1010428317736643 (*corresponding author)
- Choudhury JH, Singh AS,.…Mondal R and Ghosh SK (2015): Tobacco Carcinogen- Metabolizing Genes CYP1A1, GSTM1, and GSTT1 Polymorphisms and their Interaction with Tobacco Exposure Influence the risk of Head and Neck Cancer in Northeast Indian Population. Tumor Biology 36: 5773–5783
- Laskar RS, Talukdar FR, Choudhury JH, Singh AS, Kundu S, Dhar B, Mondal R, Ghosh SK (2015).Association of HPV with genetic and epigenetic alterations in colorectal adenocarcinoma from Indian population. Tumor Biology 36(6):4661-70)
- Ghosh SK*, Singh AS*, Mondal R*, Kapfo W, Khamo V, Singh YI (2014). Dysfunction of mitochondria due to environmental carcinogens in nasopharyngeal carcinoma in the ethnic group of Northeast Indian population. Tumor Biology. 31(1):581. (*Equal contribution)
- Laskar RS, Talukdar FR, Mondal R, Ghosh SK (2014).High frequency of young age rectal cancer in a tertiary care centre of Southern Assam, North East India. Indian Journal of Medical Research139:314-318.
- Mondal R, Ghosh SK, Talukdar FR and Laskar RS (2013). Association of mitochondrial D-loop mutations with GSTM1 and GSTT1 polymorphisms in oral carcinoma: A case control study from Northeast India. Oral oncology 49: 345-53.
- Mondal R, Ghosh SK (2013). Accumulation of mutations over the complete mitochondrial genome in tobacco-related oral cancer from northeast India. Mitochondrial DNA24(4):432-9.
- Mondal R, Ghosh SK, et al. (2013). Mitochondrial DNA Copy Number and Risk of Oral Cancer: A Report from Northeast India. PLoS ONE 8(3).
- Talukdar FR, Ghosh SK, Laskar RS, Mondal R (2013) Epigenetic, Genetic and Environmental Interactions in Esophageal Squamous Cell Carcinoma from Northeast India. PLoS ONE8(4).
- Mondal R, Ghosh SK (2013). HPV infection, GSTM1-GSTT1 genotypes, mitochondrial mutations and tobacco association with oral cancer from Northeast India. Head and Neck Oncology 5(4):46-54.
- Ghosh PR, Mahadani P, Mondal R, Das PK and Ghosh SK (2013): DNA Barcoding of Domestic Indigenous Fowls from Eastern India Environ. & Sociobiol 10 (1), 7-14
- Ghosh SK, Mondal R (2012). Quick diagnosis of female genital tuberculosis using multiplex fast polymerase chain reaction in Southern Assam, India. International Journal of Gynecology and Obstetrics118: 72–73.
- Ghosh SK, Choudhury B, Hansa J, Mondal R, Singh M, et al. (2011). Human papillomavirus testing for suspected cervical cancer patients from Southern Assam by fast-PCR. Asian Pacific Journal of Cancer Prevention12: 749–751.
Selected Conference presentations:
- Kumar M, Choudhury Y, Ghosh SK, and Mondal R: Clinical utilization of circulating cell-free DNA in identification of early somatic mutations in head and neck squamous cell carcinoma: A pilot study from Northeast India. 44th Annual Conference ISHG-2019: Genomics for Health and Precision Medicine, 30th January to 1st February 2019 Organized by National Institute of Biomedical Genomics and University of Kalyani, Kalyani, West Bengal (Best Poster Presentation Award).
- Kumar M, Choudhury Y, Ghosh SK, Mondal R: Identification of circulating cell-free mitochondrial DNA D-loop mutation in head and neck squamous cell carcinoma: A study from Northeast India. Bio-Innovation for Environmental and Health Sustainable Developments (BEHSD-2018), CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, India in association with the Biotech Research Society (BRSI), India. Lucknow Nov 2018 (Poster Presentation).
- Kumar M, Mondal R, and Ghosh SK: Circulating cell-free DNA as a non-invasive biomarker in head and neck squamous cell carcinoma: A study from Northeast India. Importance of DNA Fingerprinting. Cataloguing and Utilization of the Bioresourcees of North – East India.. Institute of Advanced Study in Science and Technology (IASST). Organized by DNA Society of India. Guwahati Nov 2017 (Best Oral Presentation Award, presented by CSIR-SRF).
- Mondal R: Association of HPV infection, xenobiotic gene polymorphism, mitochondrial mutations and tobacco with oral cancer-A study from Northeast India at Eurogin 2016, Salzburg, Austria on June 2016. (Oral presentation)
- Mondal R and Ghosh SK: Mitochondrial DNA Mutation and Copy Number in Oral Squamous Cell Carcinoma from North East Indians organized by Bose Institute, Kolkata, in2ndInternational Conference titled Perspective of Cell Signaling and Molecular Medicine, being held on January, 2012
- Mondal R and Ghosh SK: Mitochondrial DNA Genomics as Biomarker in Head and Neck Cancer from Southern Assam organized by American Association of Cancer Research (AACR) International Conference titled New Horizons in Cancer Research: Biology to Prevention to Therapy, being held on December, 2011.
- Mondal R, Hansa J, Kannan K and Ghosh SK: Detection of Mitochondrial Genome Variation in Head and Neck Cancer, 98th Indian Science Congress, January, 2011.
- Mondal R, Hansa J, Kannan R and Ghosh SK: Prevalence of Oral Cancer and its Association with Mitochondrial DNA Mutation from Northeast India, 97thIndianScience Congress, 2010 (Best poster presentation award in Medical Sciences).
- Dhar B, Mondal R, Hansa J and Ghosh SK: COLD–PCR: A Molecular Tool for Mutation Detection in TPO,BRCA1 and D-loop of mitochondrial DNA, organized by Bose Institute, Kolkata, in 2nd International Conference titled Perspective of Cell Signaling and Molecular Medicine, being held on January, 2012.
- Shirin R, Mondal R, Kannan R and Ghosh SK: Association of Human Papilloma Virus and Epstein Barr Virus in Oral and Gastrointestinal Tract carcinogenesis organized by Bose Institute in 2ndInternational Conference titled Perspective of Cell Signaling and Molecular Medicine, being held on January 8-11, 2012.
- Shirin R, Rahman F, Mondal R, Hansa J and Ghosh SK: The Effect of Betel Quid and Tobacco Chewing, GSTM1, Methylation of p16 Gene and Epstein Barr Virus (EBV) Infection on Esophageal Carcinoma: A case control study in High Incidence Region of Northeast India organized by American Association of Cancer Research (AACR) International Conference titled New Horizons in Cancer Research: Biology to Prevention to Therapy, being held on December, 2011.
Name of the book– A Textbook on DNA BARCODING (ISBN No.:-978-81-922989-4-8)
- Chapter 4-Molecular Biology in DNA Barcoding: pp-71-104
- Chapter 6-Individual DNA Barcoding: Promises and Challenges: pp-137-148
Name of the book– Computational Epigenetics and Diseases (2019)
Chapter 13 – Epigenetic Profiling in Head and Neck Cancer
Name of the book-Methods of Molecular Biology
Detection of p16 Promoter Hypermethylation by Methylation-Specific PCR. (2018)
J: Sequences on NCBI and EBML databases:
Gene bank Accession Number: (more than 50)
- Human Mitochondrial D-loop Sequence JN603607-29
- Human Mitochondrial D-loop Cancer Patient Sequence JN793569-86
- Human BRCA1gene [related to Breast Cancer] HE600032.1-38.1
Ongoing Projects As Principal Investigator
- Cell-Free Nucleic Acid as Non-Invasive Biomarker for Cancer Detection funded by Department of Science and Technology, Govt. of India under the DST INSPIRE faculty program. (2015-2020, Budget-₹35 Lakhs)
Project Sanctioned as Coordinator & Principal Investigator
- Early Detection and Comprehensive Monitoring of Head and Neck Cancer using Liquid Biopsy approach: A Study from Northeast India funded by Department of Biotechnology, Govt. of India. ( Budget- ₹58.4 Lakhs, sanctioned) Duration – three years (Sanctioned in March 2019)
To be updated….
To be updated….