Real time :
Thermal cyclers are used to amplify segments of DNA using the technique of polymerase chain reaction (PCR). Various thermal cyclers have been installed in IASST for various uses.
Real time PCR is used to amplify and simultaneously detect or quantify a targeted DNA molecule.
Gradient and non-gradient PCRThermals cyclers with/without temperature gradient facility are available in single, double and triple block formats. Single block model: Mastercycler Nexus gradient, Eppendorf Double block model: Mastercycler Nexus GX2, Eppendorf Triple block model: ProFlex PCR system, Applied Biosystems
Model: StepOnePlus real-time PCR system, Applied Biosystems