Ion-exchange chromatography

Ion-exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids. The solution to be injected is usually called a sample, and the individually separated components are called analytes.


Ion Chromatography is a useful tool for determining the presence and concentration of ions in samples. It is used for for environmental analyses such as the determination of anions (PO43-, Cl-, NO3-, etc) in surface waters.